Title

A Multiplexed Chip-Based Assay System For Investigating The Functional Development Of Human Skeletal Myotubes In Vitro

Keywords

Body-on-a-chip; Cantilevers; Functional assay; Human; Skeletal muscle

Abstract

This report details the development of a non-invasive in vitro assay system for investigating the functional maturation and performance of human skeletal myotubes. Data is presented demonstrating the survival and differentiation of human myotubes on microscale silicon cantilevers in a defined, serum-free system. These cultures can be stimulated electrically and the resulting contraction quantified using modified atomic force microscopy technology. This system provides a higher degree of sensitivity for investigating contractile waveforms than video-based analysis, and represents the first system capable of measuring the contractile activity of individual human muscle myotubes in a reliable, high-throughput and non-invasive manner. The development of such a technique is critical for the advancement of body-on-a-chip platforms toward application in pre-clinical drug development screens. © 2014 Elsevier B.V.

Publication Date

9-20-2014

Publication Title

Journal of Biotechnology

Volume

185

Number of Pages

15-18

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.jbiotec.2014.05.029

Socpus ID

84903479093 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84903479093

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