Title
Linear And Nonlinear Photophysics And Bioimaging Of An Integrin-Targeting Water-Soluble Fluorenyl Probe
Abstract
Linear photophysical characterization and two-photon absorption (2PA) properties of a new water-soluble fluorene derivative, 3-(9-(2-(2-methoxyethoxy) ethyl)-2,7-bis{3-[2-(polyethyleneglycol-550-monomethylether-1-yl)]-4-(benzo[d] thiazol-2-yl)styryl}-9H-fluoren-9-yl)propanoic acid (1), were investigated in several organic solvents and water at room temperature. A comprehensive analysis of the steady-state absorption, emission and excitation anisotropy spectra revealed electronic structures of 1, including mutual orientation of the transition dipoles, relatively weak solvatochromic effects, high fluorescence quantum yield (∼0.5-1.0), and strong aggregation in water. The 2PA spectra of 1 were obtained in the 600-900 nm spectral range by two-photon induced fluorescence (2PF) and open aperture Z-scan methods using femtosecond laser sources. No discrete 2PA bands were apparent and values of the corresponding 2PA cross sections monotonically increased in the short wavelength range up to 3000 GM in organic solvents and ∼6000 GM in aqueous solution, reflecting relatively high two-photon absorptivity. The 2PA efficiency of 1 in water increased 2-3 times relative to aprotic solvents and can be explained by cooperative electronic effects of molecular aggregates of 1 produced in aqueous media. The carboxylic acid fluorenyl probe 1 was conjugated with the cyclic peptide RGDfK. Two-photon fluorescence microscopy (2PFM) imaging of U87MG cells (and MCF-7 as control), incubated with fluorene-RGD peptide conjugate 2, demonstrated high αvβ3 integrin selectivity, making this probe particularly attractive for integrin imaging. © The Royal Society of Chemistry 2010.
Publication Date
5-28-2010
Publication Title
Organic and Biomolecular Chemistry
Volume
8
Issue
11
Number of Pages
2600-2608
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1039/b925934a
Copyright Status
Unknown
Socpus ID
77952668125 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/77952668125
STARS Citation
Morales, Alma R.; Luchita, Gheorghe; Yanez, Ciceron O.; Bondar, Mykhailo V.; and Przhonska, Olga V., "Linear And Nonlinear Photophysics And Bioimaging Of An Integrin-Targeting Water-Soluble Fluorenyl Probe" (2010). Scopus Export 2010-2014. 821.
https://stars.library.ucf.edu/scopus2010/821