Title

Dna-Rescuable Allosteric Inhibition Of Aptamer Ii Ligand Affinity By Aptamer I Element In The Shortened Vibrio Cholerae Glycine Riboswitch

Keywords

allosteric control; aptamer; cooperativity; genetic circuit; glycine riboswitch

Abstract

Glycine riboswitches contain two aptamers and turn on the expression of downstream genes in bacteria. Although full-length glycine riboswitches were shown to exhibit no glycine-binding cooperativity, the truncated glycine riboswitches were confirmed to bind two glycine molecules cooperatively. Thorough understanding of the ligand-binding cooperativity may shed light on the molecular basis of the cooperativity and help design novel intricate biosensing genetic circuits for application in synthetic biology. A previously proposed sequential model does not readily provide explanation for published data showing a deleterious mutation in the first aptamer inhibiting the glycine binding of the second one. Using the glycine riboswitch from Vibrio cholerae as a model system, we have identified a region in the first aptamer that modulates the second aptamer function especially in the shortened glycine riboswitch. Importantly, this modulation can be rescued by the addition of a complementary oligodeoxynucleotide, demonstrating the feasibility of developing this system into novel genetic circuits that sense both glycine and a DNA signal.

Publication Date

12-1-2014

Publication Title

Journal of Biochemistry

Volume

156

Issue

6

Number of Pages

323-331

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1093/jb/mvu048

Socpus ID

84941026253 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84941026253

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