Title

Regulation Of Notch 1 Signaling In Thp-1 Cells Enhances M2 Macrophage Differentiation

Keywords

Atherosclerosis; Macrophages; Monocytes; Notch 1; Polarization

Abstract

Macrophage polarization is emerging as an important area of research for the development of novel therapeutics to treat inflammatory diseases. Within the current study, the role of Notch1R in macrophage differentiation was investigated as well as downstream effects in THP-1 monocytes cultured in “inflammation mimicry” media. Interference of Notch signaling was achieved using either the pharmaceutical inhibitor DAPT or Notch1R small interfering RNA (siRNA), and Notch1R expression, macrophage phenotypes, and anti- and proinflammatory cytokine expression were evaluated. Data presented show that Notch1R expression on M1 macrophages as well as M1 macrophage differentiation is significantly elevated during cellular stress (P < 0.05). However, under identical culture conditions, interference to Notch signaling via Notch1R inhibition mitigated these results as well as promoted M2 macrophage differentiation. Moreover, when subjected to cellular stress, macrophage secretion of proinflammatory cytokines was significantly heightened (P < 0.05). Importantly, Notch1R inhibition not only diminished proinflammatory cytokine secretion but also enhanced anti-inflammatory protein release (P < 0.05). Our data suggest that Notch1R plays a pivotal role in M1 macrophage differentiation and heightened inflammatory responses. Therefore, we conclude that inhibition of Notch1R and subsequent downstream signaling enhances monocyte to M2 polarized macrophage outcomes and promotes anti-inflammatory mediation during cellular stress.

Publication Date

12-1-2014

Publication Title

American Journal of Physiology - Heart and Circulatory Physiology

Volume

307

Issue

11

Number of Pages

H1634-H1642

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1152/ajpheart.00896.2013

Socpus ID

84913554530 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84913554530

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