Title

A Differential Fluorescent Receptor For Nucleic Acid Analysis

Keywords

differential receptors; dumbbell molecular beacon; principal component analysis; SNP analysis

Abstract

Differential receptors use an array of sensors to recognize analytes. Each sensor in the array can recognize not one, but several analytes with different rates, so a single analyte triggers a response of several sensors in the array. The receptor thus produces a pattern of signals that is unique for each analyte, thereby enabling identification of a specific analyte by producing a "fingerprint" pattern. We applied this approach for the analysis of DNA sequences of Mycobacterium tuberculosis strains that differ by single nucleotide substitutions in the 81-bp hot-spot region that imparts rifampin resistance. The technology takes advantage of the new multicomponent, selfassembling sensor, which produces a fluorescent signal in the presence of specific DNA sequences. A differential fluorescent receptor (DFR) contained an array of three such sensors and differentiated at least eight DNA sequences. The approach requires only one molecular-beacon-like fluorescent reporter, which can be used by all three sensors. The DFR developed in this study represents a cost-efficient alternative to molecular diagnostic technologies that use fluorescent hybridization probes. Make it glow: SNP-tolerant sensors were developed by using a differential fluorescent receptor (DFR) approach, which requires only one fluorescent reporter to differentiate between eight or more analytes. This strategy enabled differentiation between drug-resistant and drug-susceptible Mycobacterium tuberculosis strains and shows great clinical potential for multiple diseases. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Publication Date

1-24-2014

Publication Title

ChemBioChem

Volume

15

Issue

2

Number of Pages

228-231

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/cbic.201300657

Socpus ID

84892798562 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84892798562

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