Title

Functional And Structural Analysis Of Mice Trpc6 With Human Analogue Through Homology Modelling

Keywords

3D structure; Glioblastoma; Homology modelling; TPRC6

Abstract

Homology models are increasingly used to determine structural and functional relationships of genes and proteins in biomedical research. In the current study, for the first time, we compared the TRPC6 gene in mouse and human. The protein encoded by this gene forms a receptor activated calcium channel in cell membrane. Defects in this gene have been implicated in a wide range of diseases including glioblastomas. To determine the structural similarities in mouse and human TRPC6, we used standard bioinformatics tools such as fold prediction to identify the protein 3D structure, sequence-structure comparison, and prediction of template and protein structure. We also used glioblastoma cell line U373MG and human glioblastoma tumour tissues to study the expression of TRPC6 in disease conditions to implicate this gene in pathological ailment. Based on the results we conclude that human TRPC6 contains 90% identity and 93% similarity with mouse TRPC6, suggesting that this protein is well conserved in these two species. These isoforms likely demonstrate similar mechanisms in regulating gene expression; thus TRPC6 studies in mice may be extrapolated to humans. Copyright © 2014 Inderscience Enterprises Ltd.

Publication Date

1-1-2014

Publication Title

International Journal of Bioinformatics Research and Applications

Volume

10

Issue

2

Number of Pages

206-216

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1504/IJBRA.2014.059536

Socpus ID

85047687080 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85047687080

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