Title

Characterizing The Dynamics Of The Leader-Linker Interaction In The Glycine Riboswitch With Site-Directed Spin Labeling

Abstract

Site-directed spin labeling with continuous wave electron paramagnetic resonance (EPR) spectroscopy was utilized to characterize dynamic features of the kink-turn motif formed through a leader-linker interaction in the Vibrio cholerae glycine riboswitch. Efficient incorporation of spin-labels into select sites within the phosphate backbone of the leader-linker region proceeded via splinted ligation of chemically synthesized spin-labeled oligonucleotides to in vitro transcribed larger RNA fragments. The resultant nitroxide EPR line shapes have spectral characteristics consistent with a kink-turn motif and reveal differential backbone dynamics that are modulated by the presence of magnesium, potassium, and glycine. © 2014 American Chemical Society.

Publication Date

6-10-2014

Publication Title

Biochemistry

Volume

53

Issue

22

Number of Pages

3526-3528

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/bi500404b

Socpus ID

84902197971 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84902197971

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