Title

Aurora A Kinase Modulates Actin Cytoskeleton Through Phosphorylation Of Cofilin: Implication In The Mitotic Process

Keywords

Actin cytoskeleton; Aurora A kinase; Cofilin; Mitosis; Phosphorylation

Abstract

Aurora A kinase regulates early mitotic events through phosphorylation and activation of a variety of proteins. Specifically, Aur-A is involved in centrosomal separation and formation of mitotic spindles in early prophase. The effect of Aur-A on mitotic spindles is mediated by the modulation of microtubule dynamics and association with microtubule binding proteins. In this study we show that Aur-A exerts its effects on spindle organization through the regulation of the actin cytoskeleton. Aurora A phosphorylates Cofilin at multiple sites including S3 resulting in the inactivation of its actin depolymerizing function. Aur-A interacts with Cofilin in early mitotic phases and regulates its phosphorylation status. Cofilin phosphorylation follows a dynamic pattern during the progression of prophase to metaphase. Inhibition of Aur-A activity induced a delay in the progression of prophase to metaphase. Aur-A inhibitor also disturbed the pattern of Cofilin phosphorylation, which correlated with the mitotic delay. Our results establish a novel function of Aur-A in the regulation of actin cytoskeleton reorganization, through Cofilin phosphorylation during early mitotic stages. © 2014 Elsevier B.V.

Publication Date

1-1-2014

Publication Title

Biochimica et Biophysica Acta - Molecular Cell Research

Volume

1843

Issue

11

Number of Pages

2719-2729

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.bbamcr.2014.07.014

Socpus ID

84905962529 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84905962529

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