Title

Cationic Peptides Neutralize Ox-Ldl, Prevent Its Uptake By Macrophages, And Attenuate Inflammatory Response

Keywords

Atherosclerosis; Lipid peroxides; Lysine; Modified lipoproteins; Oxidation

Abstract

Objective: Apolipoprotein A1 (ApoA1) and apolipoprotein E (ApoE) mimetic peptides have attracted attention due to their ability to reduce atherosclerosis and exhibit antioxidant, anti-inflammatory, and hypolipidemic properties. In this study, we tested whether three distinct and unrelated cationic peptides would inhibit the oxidation of lipoproteins and whether they would counteract and neutralize the negatively charged modified lipoproteins, inhibit their uptake and inflammation by macrophages. Methods and results: 5F-mimetic peptide of ApoA1, LL27 derived from the anti-microbial peptide hCAP, and a human glycodelin derived peptide were commercially synthesized. We noted that these three distinct cationic lysine-rich peptides, two of which were unrelated to any known apolipoproteins, inhibited copper-mediated oxidation of lipoproteins and reduced lipid peroxides in a lysine dependent manner. The peptides also retarded the electrophoretic mobility of previously oxidized LDL and acetylated LDL by virtue of their net positive charge. Pre-incubation of peptides with modified lipoproteins reduced the uptake of the latter by macrophages, thus preventing the formation of foam cells. The cationic peptides inhibited oxidized LDL (Ox-LDL)-induced inflammatory response both invitro and invivo. Conclusion: Based on these results, we suggest that in addition to the well known mimetic peptides, other suitable cationic peptides may be of use for controlling Ox-LDL mediated inflammation and atherosclerotic progression. © 2014 Elsevier Ireland Ltd.

Publication Date

1-1-2014

Publication Title

Atherosclerosis

Volume

236

Issue

1

Number of Pages

-

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.atherosclerosis.2014.06.020

Socpus ID

84905828387 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84905828387

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