Photodynamic Therapy With Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers

Keywords

Cancer; Cell culture; Chemistry; Conducting polymer; Fluorescence microscopy; Issue 104; MTT; Photodynamic therapy

Abstract

In this article a method for the fabrication and reproducible in-vitro evaluation of conducting polymer nanoparticles blended with fullerene as the next generation photosensitizers for Photodynamic Therapy (PDT) is reported. The nanoparticles are formed by hydrophobic interaction of the semiconducting polymer MEH-PPV (poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene]) with the fullerene PCBM (phenyl-C61-butyric acid methyl ester) in the presence of a non-compatible solvent. MEH-PPV has a high extinction coefficient that leads to high rates of triplet formation, and efficient charge and energy transfer to the fullerene PCBM. The latter processes enhance the efficiency of the PDT system through fullerene assisted triplet and radical formation, and ultrafast deactivation of MEH-PPV excited stated. The results reported here show that this nanoparticle PDT sensitizing system is highly effective and shows unexpected specificity to cancer cell lines.

Publication Date

10-28-2015

Publication Title

Journal of Visualized Experiments

Volume

2015

Issue

104

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.3791/53038

Socpus ID

84946426566 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84946426566

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