Rgd-Conjugated Two-Photon Absorbing Near-Ir Emitting Fluorescent Probes For Tumor Vasculature Imaging

Abstract

Observation of the activation and inhibition of angiogenesis processes is important in the progression of cancer. Application of targeting peptides, such as a small peptide that contains adjacent l-arginine (R), glycine (G) and l-aspartic acid (D) residues can afford high selectivity and deep penetration in vessel imaging. To facilitate deep tissue vasculature imaging, probes that can be excited via two-photon absorption (2PA) in the near-infrared (NIR) and subsequently emit in the NIR are essential. In this study, the enhancement of tissue image quality with RGD conjugates was investigated with new NIR-emitting pyranyl fluorophore derivatives in two-photon fluorescence microscopy. Linear and nonlinear photophysical properties of the new probes were comprehensively characterized; significantly the probes exhibited good 2PA over a broad spectral range from 700-1100 nm. Cell and tissue images were then acquired and examined, revealing deep penetration and high contrast with the new pyranyl RGD-conjugates up to 350 μm in tumor tissue.

Publication Date

1-1-2015

Publication Title

Organic and Biomolecular Chemistry

Volume

13

Issue

43

Number of Pages

10716-10725

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1039/c5ob01536g

Socpus ID

84946606003 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84946606003

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