In Vitro Analysis Of The Very-Low Density Lipoprotein Export From The Trans-Golgi Network

Keywords

Apolipoprotein B; Endoplasmic reticulum; Post-golgi VLDL transport vesicle (PG-VTV); Trans-Golgi network (TGN); Triacylglycerol; Very low-density lipoprotein (VLDL)

Abstract

The movement of mature VLDL particles from the TGN to the plasma membrane (PM) is a complex physiological process that plays a critical role in hepatic lipid homeostasis. However, the molecular mechanisms regulating these intracellular transport events had not been studied until recently because of the lack of appropriate molecular assays and techniques. This unit provides a detailed description of cell-free approaches and techniques to study the TGNto- PM transport of the mature VLDL at the molecular level. A major emphasis is placed on the preparation and purification of sub-cellular organelles because the success of in vitro assays for the vesicle formation and fusion depends on the quality of the isolated TGN, hepatic PM and hepatic cytosol. A number of critical factors that control the formation of mature VLDL-containing vesicle, the PG-VTV, from the TGN and their subsequent targeting to and fusion with the hepatic PM have been discussed.

Publication Date

1-1-2015

Publication Title

Current Protocols in Cell Biology

Volume

2015

Number of Pages

11.21.1-11.21.17

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/0471143030.cb1121s67

Socpus ID

84949238994 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84949238994

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