Split Spinach Aptamer For Highly Selective Recognition Of Dna And Rna At Ambient Temperatures

Keywords

aptameric sensor; high selectivity; label-free probes; RNA; split probes

Abstract

Split spinach aptamer (SSA) probes for fluorescent analysis of nucleic acids were designed and tested. In SSA design, two RNA or RNA/DNA strands hybridized to a specific nucleic acid analyte and formed a binding site for low-fluorescent 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) dye, which resulted in up to a 270-fold increase in fluorescence. The major advantage of the SSA over state-of-the art fluorescent probes is high selectivity: it produces only background fluorescence in the presence of a single-base-mismatched analyte, even at room temperature. SSA is therefore a promising tool for label-free analysis of nucleic acids at ambient temperatures.

Publication Date

9-2-2016

Publication Title

ChemBioChem

Number of Pages

1589-1592

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/cbic.201600323

Socpus ID

84985997674 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84985997674

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