Multiplexed Labeling Of Genomic Loci With Dcas9 And Engineered Sgrnas Using Crisprainbow

Abstract

A lack of techniques to image multiple genomic loci in living cells has limited our ability to investigate chromosome dynamics. Here we describe CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of fluorescent proteins. We demonstrate simultaneous imaging of up to six chromosomal loci in individual live cells and document large differences in the dynamic properties of different chromosomal loci.

Publication Date

5-1-2016

Publication Title

Nature Biotechnology

Volume

34

Issue

5

Number of Pages

528-530

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1038/nbt.3526

Socpus ID

84966908028 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84966908028

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