Dna Damage And Repair In Forensic Science

Keywords

Active pixel sensor; Charge-coupled device; Complementary metal-oxide semiconductor; Fluorescent lifetime imaging microscopy; Retinal prosthesis; Single-photon avalanche diode; Voltage-sensitive dye

Abstract

The principal concern from the forensic science standpoint is that many of the environmentally induced lesions are expected to be inhibitory toward DNA-polymerase mediated primer extension and may result in amplification, and hence DNA typing, failure. To understand these processes, it is beneficial to consider the mechanisms by which DNA damage may take place. The ability to detect DNA polymorphisms using molecular genetic techniques has revolutionized the forensic analysis of biological evidence. The major pathways involved in the correction of DNA damage include base excision repair (BER), nucleotide excision repair (NER), single strand break repair (SSBR), mismatch repair (MMR), direct reversal with the photoreactivation activity of photolyases, by recombination and rejoining pathways, or through tolerance with translesion synthesis (TLS). This chapter explores the types of DNA damage that predominate in physiological stains deposited at a crime scene.

Publication Date

3-11-2016

Publication Title

Forensic Science: A Multidisciplinary Approach

Number of Pages

193-214

Document Type

Article; Book Chapter

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/9783527693535.ch10

Socpus ID

85017446657 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85017446657

This document is currently not available here.

Share

COinS