Human Organ Tissue Identification By Targeted Rna Deep Sequencing To Aid The Investigation Of Traumatic Injury

Keywords

Forensic science; Human organ tissue; Massively parallel sequencing; MRNA; Tissue identification

Abstract

Molecular analysis of the RNA transcriptome from a putative tissue fragment should permit the assignment of its source to a specific organ, since each will exhibit a unique pattern of gene expression. Determination of the organ source of tissues from crime scenes may aid in shootings and other investigations. We have developed a prototype massively parallel sequencing (MPS) mRNA profiling assay for organ tissue identification that is designed to definitively identify 10 organ/tissue types using a targeted panel of 46 mRNA biomarkers. The identifiable organs and tissues include brain, lung, liver, heart, kidney, intestine, stomach, skeletal muscle, adipose, and trachea. The biomarkers were chosen after iterative specificity testing of numerous candidate genes in various tissue types. The assay is very specific, with little cross-reactivity with non-targeted tissue, and can detect RNA mixtures from different tissues. We also demonstrate the ability of the assay to successful identify the tissue source of origin using a single blind study.

Publication Date

11-10-2017

Publication Title

Genes

Volume

8

Issue

11

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.3390/genes8110319

Socpus ID

85034049258 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85034049258

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