Title

Complete Genome Sequence Of Lytic Bacteriophage Rg-2014 That Infects The Multidrug Resistant Bacterium Delftia Tsuruhatensis Arb-1

Keywords

Bacteriophage; Biofilm; Biofouling; Delftia tsuruhatensis; Genome; Multidrug resistant; Podoviridae

Abstract

A lytic bacteriophage RG-2014 infecting a biofilm forming multidrug resistant bacterium Delftia tsuruhatensis strain ARB-1 as its host was isolated from a full-scale municipal wastewater treatment plant. Lytic phage RG-2014 was isolated for developing phage based therapeutic approaches against Delftia tsuruhatensis strain ARB-1. The strain ARB-1 belongs to the Comamonadaceae family of the Betaproteobacteria class. RG-2014 was characterized for its type, burst size, latent and eclipse time periods of 150±9 PFU/cell, 10-min, <5-min, respectively. The phage was found to be a dsDNA virus belonging to the Podoviridae family. It has an isometric icosahedrally shaped capsid with a diameter of 85 nm. The complete genome of the isolated phage was sequenced and determined to be 73.8 kbp in length with a G+C content of 59.9%. Significant similarities in gene homology and order were observed between Delftia phage RG-2014 and the E. coli phage N4 indicating that it is a member of the N4-like phage group.

Publication Date

12-18-2017

Publication Title

Standards in Genomic Sciences

Volume

12

Issue

1

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1186/s40793-017-0290-y

Socpus ID

85038370958 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85038370958

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