A Phenotypic Invitro Model For The Main Determinants Of Human Whole Heart Function

Keywords

Cardiomyocyte; Conduction; Force generation; Functional assay; Human; Serum-free

Abstract

This article details the construction and testing of a phenotypic assay system that models invivo cardiac function in a parallel invitro environment with human stem cell derived cardiomyocytes. The major determinants of human whole-heart function were experimentally modeled by integrating separate 2D cellular systems with BioMicroelectromechanical Systems (BioMEMS) constructs. The model features a serum-free defined medium to enable both acute and chronic evaluation of drugs and toxins. The integration of data from both systems produced biologically relevant predictions of cardiac function in response to varying concentrations of selected drugs. Sotalol, norepinephrine and verapamil were shown to affect the measured parameters according to their specific mechanism of action, in agreement with clinical data. This system is applicable for cardiac side effect assessment, general toxicology, efficacy studies, and evaluation of invitro cellular disease models in body-on-a-chip systems.

Publication Date

8-1-2015

Publication Title

Biomaterials

Volume

60

Number of Pages

20-30

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.biomaterials.2015.04.035

Socpus ID

84930434509 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84930434509

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