A Mutation-Resistant Deoxyribozyme Or Gate For Highly Selective Detection Of Viral Nucleic Acids

Abstract

Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases.

Publication Date

1-1-2017

Publication Title

Chemical Communications

Volume

53

Issue

76

Number of Pages

10592-10595

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1039/c7cc05576e

Socpus ID

85029855915 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85029855915

This document is currently not available here.

Share

COinS