Cytochrome C Oxidase Subunit 1-Based Human Rna Quantification To Enhance Mrna Profiling In Forensic Biology

Keywords

Cytochrome c oxidase subunit I; forensic science; quantitative polymerase chain reaction; RNA quantification

Abstract

RNA analysis offers many potential applications in forensic science, and molecular identification of body fluids by analysis of cell-specific RNA markers represents a new technique for use in forensic cases. However, due to the nature of forensic materials that often admixed with nonhuman cellular components, human-specific RNA quantification is required for the forensic RNA assays. Quantification assay for human RNA has been developed in the present study with respect to body fluid samples in forensic biology. The quantitative assay is based on real-time reverse transcription-polymerase chain reaction of mitochondrial RNA cytochrome c oxidase subunit I and capable of RNA quantification with high reproducibility and a wide dynamic range. The human RNA quantification improves the quality of mRNA profiling in the identification of body fluids of saliva and semen because the quantification assay can exclude the influence of nonhuman components and reduce the adverse affection from degraded RNA fragments.

Publication Date

7-1-2017

Publication Title

Journal of Forensic Science and Medicine

Volume

3

Issue

3

Number of Pages

115-121

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.4103/jfsm.jfsm_63_17

Socpus ID

85090248144 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85090248144

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