Endogenous Alpha-Synuclein Protein Analysis From Human Brain Tissues Using Single-Molecule Pull-Down Assay

Abstract

Alpha-synuclein (α-SYN) is a central molecule in Parkinson's disease pathogenesis. Despite several studies, the molecular nature of endogenous α-SYN especially in human brain samples is still not well understood due to the lack of reliable methods and the limited amount of biospecimens. Here, we introduce α-SYN single-molecule pull-down (α-SYN SiMPull) assay combined with in vivo protein crosslinking to count individual α-SYN protein and assess its native oligomerization states from biological samples including human postmortem brains. This powerful single-molecule assay can be highly useful in diagnostic applications using various specimens for neurodegenerative diseases including Alzheimer's disease and Parkinson's disease.

Publication Date

12-19-2017

Publication Title

Analytical Chemistry

Volume

89

Issue

24

Number of Pages

13044-13048

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/acs.analchem.7b04335

Socpus ID

85037553245 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85037553245

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