Normal-Phase Liquid Chromatography Retention Behavior Of Polycyclic Aromatic Hydrocarbon And Their Methyl-Substituted Derivatives On An Aminopropyl Stationary Phase

Keywords

Non-planarity; Normal-phase liquid chromatography; Polycyclic aromatic hydrocarbons; Retention index

Abstract

Retention indices for 124 polycyclic aromatic hydrocarbons (PAHs) and 62 methyl-substituted (Me-) PAHs were determined using normal-phase liquid chromatography (NPLC) on a aminopropyl (NH2) stationary phase. PAH retention behavior on the NH2 phase is correlated to the total number of aromatic carbons in the PAH structure. Within an isomer group, non-planar isomers generally elute earlier than planar isomers. MePAHs generally elute slightly later but in the same region as the parent PAHs. Correlations between PAH retention behavior on the NH2 phase and PAH thickness (T) values were investigated to determine the influence of non-planarity for isomeric PAHs with four to seven aromatic rings. Correlation coefficients ranged from r = 0.19 (five-ring peri-condensed molecular mass (MM) 252 Da) to r = −0.99 (five-ring cata-condensed MM 278 Da). In the case of the smaller PAHs (MM ≤ 252 Da), most of the PAHs had a planar structure and provided a low correlation. In the case of larger PAHs (MM ≥ 278 Da), nonplanarity had a significant influence on the retention behavior and good correlation between retention and T was obtained for the MM 278 Da, MM 302 Da, MM 328 Da, and MM 378 Da isomer sets. [Figure not available: see fulltext.].

Publication Date

9-1-2017

Publication Title

Analytical and Bioanalytical Chemistry

Volume

409

Issue

22

Number of Pages

5291-5305

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/s00216-017-0474-8

Socpus ID

85024472997 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85024472997

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