Title
Resonant Waveguide Grating Imager For Single Cell Monitoring Of The Invasion Of 3D Speheroid Cancer Cells Through Matrigel
Keywords
Adhesion; Colorectal cancer cell; Dynamic mass redistribution; Extracellular matrix; Invasion; Migration; Multicellular spheroid; PTEN; Resonant waveguide grating
Abstract
The invasion of cancer cells through their surrounding extracellular matrices is the first critical step to metastasis, a devastating event to cancer patients. However, in vitro cancer cell invasion is mostly studied using two-dimensional (2D) models. Three-dimensional (3D) multicellular spheroids may offer an advantageous cell model for cancer research and oncology drug discovery. This chapter describes a label-free, realtime, and single-cell approach to quantify the invasion of 3D spheroid colon cancer cells through Matrigel using a spatially resolved resonant waveguide grating imager.
Publication Date
1-1-2017
Publication Title
Methods in Molecular Biology
Volume
1571
Number of Pages
143-160
Document Type
Article; Book Chapter
Personal Identifier
scopus
DOI Link
https://doi.org/10.1007/978-1-4939-6848-0_10
Copyright Status
Unknown
Socpus ID
85015099588 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/85015099588
STARS Citation
Febles, Nicole K.; Chandrasekaran, Siddarth; and Fang, Ye, "Resonant Waveguide Grating Imager For Single Cell Monitoring Of The Invasion Of 3D Speheroid Cancer Cells Through Matrigel" (2017). Scopus Export 2015-2019. 6415.
https://stars.library.ucf.edu/scopus2015/6415