Resonant Waveguide Grating Imager For Single Cell Monitoring Of The Invasion Of 3D Speheroid Cancer Cells Through Matrigel

Keywords

Adhesion; Colorectal cancer cell; Dynamic mass redistribution; Extracellular matrix; Invasion; Migration; Multicellular spheroid; PTEN; Resonant waveguide grating

Abstract

The invasion of cancer cells through their surrounding extracellular matrices is the first critical step to metastasis, a devastating event to cancer patients. However, in vitro cancer cell invasion is mostly studied using two-dimensional (2D) models. Three-dimensional (3D) multicellular spheroids may offer an advantageous cell model for cancer research and oncology drug discovery. This chapter describes a label-free, realtime, and single-cell approach to quantify the invasion of 3D spheroid colon cancer cells through Matrigel using a spatially resolved resonant waveguide grating imager.

Publication Date

1-1-2017

Publication Title

Methods in Molecular Biology

Volume

1571

Number of Pages

143-160

Document Type

Article; Book Chapter

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/978-1-4939-6848-0_10

Socpus ID

85015099588 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85015099588

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