Title
A Cell-Based Fluorescent Assay To Detect The Activity Of Ab Toxins That Inhibit Protein Synthesis
Keywords
AB toxin; Ricin; Shiga toxin; Toxicity assay; Toxin detection; Toxin inhibitors; Vero cells
Abstract
Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.
Publication Date
1-1-2017
Publication Title
Methods in Molecular Biology
Volume
1600
Number of Pages
25-36
Document Type
Article; Book Chapter
Personal Identifier
scopus
DOI Link
https://doi.org/10.1007/978-1-4939-6958-6_3
Copyright Status
Unknown
Socpus ID
85019159136 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/85019159136
STARS Citation
Cherubin, Patrick; Quiñones, Beatriz; Elkahoui, Salem; Yokoyama, Wallace; and Teter, Ken, "A Cell-Based Fluorescent Assay To Detect The Activity Of Ab Toxins That Inhibit Protein Synthesis" (2017). Scopus Export 2015-2019. 6461.
https://stars.library.ucf.edu/scopus2015/6461