A Cell-Based Fluorescent Assay To Detect The Activity Of Ab Toxins That Inhibit Protein Synthesis

Creator

Patrick Cherubin, University of Central Florida
Beatriz Quiñones, USDA ARS Western Regional Research Center (WRRC)
Salem Elkahoui, Centre de Biotechnologie de Borj Cédria
Wallace Yokoyama, USDA ARS Western Regional Research Center (WRRC)
Ken Teter, University of Central Florida

Keywords

AB toxin; Ricin; Shiga toxin; Toxicity assay; Toxin detection; Toxin inhibitors; Vero cells

Abstract

Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.

Publication Date

1-1-2017

Publication Title

Methods in Molecular Biology

Volume

1600

Number of Pages

25-36

Document Type

Article; Book Chapter

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/978-1-4939-6958-6_3

Copyright Status

Unknown

Socpus ID

85019159136 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85019159136

STARS Citation

Cherubin, Patrick; Quiñones, Beatriz; Elkahoui, Salem; Yokoyama, Wallace; and Teter, Ken, "A Cell-Based Fluorescent Assay To Detect The Activity Of Ab Toxins That Inhibit Protein Synthesis" (2017). Scopus Export 2015-2019. 6461.
https://stars.library.ucf.edu/scopus2015/6461