Exploitation Of Mangrove Endophytic Fungi For Infectious Disease Drug Discovery
Keywords
Endophytic fungi; Epigenetic modification; Infectious disease drug discovery; Mangroves; Screening
Abstract
There is an acute need for new and effective agents to treat infectious diseases. We conducted a screening program to assess the potential of mangrove-derived endophytic fungi as a source of new antibiotics. Fungi cultured in the presence and absence of small molecule epigenetic modulators were screened against Mycobacterium tuberculosis and the ESKAPE panel of bacterial pathogens, as well as two eukaryotic infective agents, Leishmania donovani and Naegleria fowleri. By comparison of bioactivity data among treatments and targets, trends became evident, such as the result that more than 60% of active extracts were revealed to be selective to a single target. Validating the technique of using small molecules to dysregulate secondary metabolite production pathways, nearly half (44%) of those fungi producing active extracts only did so following histone deacetylase inhibitory (HDACi) or DNA methyltransferase inhibitory (DNMTi) treatment.
Publication Date
10-10-2018
Publication Title
Marine Drugs
Volume
16
Issue
10
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.3390/md16100376
Copyright Status
Unknown
Socpus ID
85054732331 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/85054732331
STARS Citation
Demers, Danielle H.; Knestrick, Matthew A.; Fleeman, Renee; Tawfik, Rahmy; and Azhari, Ala, "Exploitation Of Mangrove Endophytic Fungi For Infectious Disease Drug Discovery" (2018). Scopus Export 2015-2019. 8275.
https://stars.library.ucf.edu/scopus2015/8275