Elevated And Correlated Expressions Of Mir-24, Mir-30D, Mir-146A, And Sfrp-4 In Human Abdominal Adipose Tissue Play A Role In Adiposity And Insulin Resistance

Abstract

Objective. We explored the relationships among microRNAs (miRNAs) and SFRP4, as they relate to adipose tissue functions including lipolysis, glucose and glycerol turnover, and insulin sensitivity. Methods. Abdominal adipose tissue (AbdAT) levels of thirteen microRNAs (miRNAs), SFRP4, and VEGF in lean nondiabetic subjects (n = 7), subjects with obesity (n = 5), and subjects with obesity and type 2 diabetes (T2DM) (n = 5) were measured by qPCR. Insulin sensitivity was measured by the euglycemic-hyperinsulinemic clamp. Osmium fixation and Coulter counting were used for adipocyte sizing. Data were analyzed using generalized linear models that adjusted for age, gender, and ethnicity. Results. AbdAT miR-24, miR-30d, and miR-146a were elevated in subjects with obesity (P < 0 05) and T2DM (P < 0 1) and positively correlated with measures of percent body fat by DXA (rmiR 24 = 0 894, rmiR 146a = 0 883, P < 0 05), and AbdAT SFRP4 (rmiR 30 = 0 93, rmiR 146a = 0 88, P < 0 05). These three miRNAs additionally correlated among themselves (rmiR 24~miR 146a = 0 90, rmiR 30~miR 146a = 0 85, P < 0 01). Conclusions. This study suggests a novel association between the elevated levels of miRNAs miR-24, miR-30d, and miR-146a (apparently coregulated) and the level of SFRP4 transcript in AbdAT of subjects with obesity and T2DM. These molecules might be part of a regulatory loop involved in AbdAT remodeling/adiposity and systemic insulin resistance.

Publication Date

1-1-2018

Publication Title

International Journal of Endocrinology

Volume

2018

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1155/2018/7351902

Socpus ID

85055635833 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85055635833

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