Extended Field-Of-View Single-Molecule Imaging By Highly Inclined Swept Illumination

Abstract

Single-molecule imaging inside cells is a valuable tool to study subcellular structures, gene expression, and the dynamics of biomolecules. Here, we present highly inclined swept tile illumination microscopy. By sweeping a thin highly inclined and laminated optical sheet (HILO) with confocal slit detection, our method provides a twofold thinner illumination and greater than fortyfold larger imaging area than conventional HILO microscopy, enabling 3D single-molecule imaging with a high signal-to-background ratio. We demonstrate single-molecule mRNA imaging with a few probes or a single probe in cultured cells and mouse brain tissues, and video-rate live-cell imaging.

Publication Date

9-20-2018

Publication Title

Optica

Volume

5

Issue

9

Number of Pages

1063-1069

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1364/OPTICA.5.001063

Socpus ID

85053852972 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85053852972

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