Faptasyme: A Strategy For Converting A Monomer/ Oligomer-Nonselective Aptameric Sensor Into An Oligomer-Selective One

Keywords

Aptamers; Aptazymes; Biosensors; Deoxyribozymes; Split probes

Abstract

Aptameric sensors can bind molecular targets and produce output signals, a phenomenon that is used in bioassays. In some cases, it is important to distinguish between monomeric and oligomeric forms of a target. Here, we propose a strategy to convert a monomer/oligomer-nonselective sensor into an oligomer-selective sensor. We designed an aptazyme that produced a high fluorescent output in the presence of oligomeric α-synuclein (a molecular marker of Parkinson’s disease) but not its monomeric form. The strategy is potentially useful in the design of point-of-care tests for the diagnosis of neurodege-nerative diseases.

Publication Date

6-4-2018

Publication Title

ChemBioChem

Volume

19

Issue

11

Number of Pages

1123-1126

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/cbic.201800017

Socpus ID

85046007711 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85046007711

This document is currently not available here.

Share

COinS