Title

Oleuropein Is Responsible For The Major Anti-Inflammatory Effects Of Olive Leaf Extract

Keywords

gallic acid; OLE; oleuropein; olive leaf extract; phenolic compounds; TNFa

Abstract

Olive leaves are rich in polyphenolic compounds that are known to have antioxidant, antimicrobial, and anti-inflammatory activities. Therefore, olive leaf extract (OLE) is considered as a natural supplement. In this study we evaluated the antibacterial and the anti-inflammatory effect of OLE and its individual phenolic components in vitro. Polymorphonuclear cells (PMNCs) were isolated from the whole blood using Histopaque solution and cultured in RPMI-enriched medium. Tumor necrosis factor α (TNFα) level was determined by ELISA after 24 h of lipopolysaccharide stimulation. The antibacterial activity of OLE was determined by well diffusion assay. We found a significant decrease in TNFα secretion level in PMNCs culture treated with OLE. Oleuropein is the only OLE component that has shown anti-inflammatory effects at a concentration of 20 μg/mL. Furthermore, OLE exhibited antibacterial activity against some gram positive bacterial strains; however, gram negative bacterial strains were resistant to OLE. Downregulation of TNFα secretion in PMNCs culture in response to OLE treatment indicates that this polyphenol-rich extract has an anti-inflammatory effect, and oleuropein is the major OLE component responsible for this effect. The antibacterial activity of OLE is limited to gram positive bacteria.

Publication Date

3-1-2018

Publication Title

Journal of Medicinal Food

Volume

21

Issue

3

Number of Pages

302-305

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1089/jmf.2017.0070

Socpus ID

85044663457 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85044663457

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