Cations Modulate Actin Bundle Mechanics, Assembly Dynamics, And Structure

Abstract

Actin bundles are key factors in the mechanical support and dynamic reorganization of the cytoskeleton. High concentrations of multivalent counterions promote bundle formation through electrostatic attraction between actin filaments that are negatively charged polyelectrolytes. In this study, we evaluate how physiologically relevant divalent cations affect the mechanical, dynamic, and structural properties of actin bundles. Using a combination of total internal reflection fluorescence microscopy, transmission electron microscopy, and dynamic light scattering, we demonstrate that divalent cations modulate bundle stiffness, length distribution, and lateral growth. Molecular dynamics simulations of an all-atom model of the actin bundle reveal specific actin residues coordinate cation-binding sites that promote the bundle formation. Our work suggests that specific cation interactions may play a fundamental role in the assembly, structure, and mechanical properties of actin bundles.

Publication Date

4-12-2018

Publication Title

Journal of Physical Chemistry B

Volume

122

Issue

14

Number of Pages

3826-3835

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/acs.jpcb.8b00663

Socpus ID

85045473791 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85045473791

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