Identification And Evaluation Of Anti-Inflammatory Properties Of Aqueous Components Extracted From Sesame (Sesamum Indicum) Oil

Keywords

Inflammation; Liquid chromatography; Mass spectrometry; Metabolomics; Methoxyphenol derivatives

Abstract

We previously reported that sesame oil (SO) has anti-inflammatory, anti-atherosclerotic and lipid lowering properties in vivo. Our recent studies have shown that, an aqueous extract of sesame oil (SOAE) has also anti-inflammatory and anti-atherosclerotic properties but with no lipid lowering effects. The extent of reduction in atherosclerosis led us to identify components of SOAE and evaluate their anti-inflammatory properties in vitro. Liquid chromatography mass spectrometric method was used to detect and identify components of SOAE. Methoxyphenol derivatives, short and long chain carboxylic acids, dicarboxylic acids, hydroxy and oxo- carboxylic acids were detected. To our surprise, sesamol and its derivatives (lignans), were not present in the SOAE. Among the identified, a combination of methoxy phenol compounds were selected and tested their ability to reduce LPS induced inflammatory gene expression. Monocyte derived macrophages/RAW 264.7 macrophages were pre-treated with these compounds for 2 h, followed by LPS stimulation for 24 h and pro-inflammatory gene expressions were analyzed. These methoxyphenol derivatives showed potent anti-inflammatory properties. In conclusion, the anti-inflammatory molecules associated with SO may contribute the anti-inflammatory and anti-atherosclerotic properties. Also, our results shed light for the development of SOAE based non-pharmacological therapeutics, nutritional supplements and health products for various inflammatory diseases in the future.

Publication Date

6-15-2018

Publication Title

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volume

1087-1088

Number of Pages

61-69

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.jchromb.2018.04.029

Socpus ID

85046103130 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85046103130

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