Title

Reelin expression is upregulated following ocular tissue injury

Authors

Authors

J. S. Pulido; I. Sugaya; J. Comstock;K. Sugaya

Comments

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Abbreviated Journal Title

Graefes Arch. Clin. Exp. Ophthalmol.

Keywords

cornea; migration; regeneration; retina; stem cell; reelin; NEURAL STEM-CELLS; ADULT-MOUSE; DIFFERENTIATION; NEURONS; Ophthalmology

Abstract

Purpose Reelin is important in the guidance of neuronal stem cells in the central nervous system during normal development. We wished to determine whether reelin is expressed in the retina and cornea after injury. Methods Mice underwent laceration of their retina as well as corneal epithelial debridement. The mice were sacrificed at 3 days, and eyes were fixed and stained for reelin expression and reelin messenger ribonucleic acid (mRNA). Results In normal eyes, reelin was expressed only at very low levels in the ganglion cell layer of the retina and the endothelial cell layer of the cornea. In injured eyes, there was marked expression in reelin immunoreactivity in the retina and cornea. Reelin gene expression was seen in the retina and cornea. Conclusions Reelin is expressed during normal retinogenesis. This study shows that reelin is also upregulated following injury to the retina and cornea. The expression of reelin following injury suggests that reelin may play an important role in regulating stem cell trafficking in neuronal and nonneuronal tissues following injury similar to its role in normal organogenesis.

Journal Title

Graefes Archive for Clinical and Experimental Ophthalmology

Volume

245

Issue/Number

6

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

889

Last Page

893

WOS Identifier

WOS:000246594000017

ISSN

0721-832X

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