Reelin expression is upregulated following ocular tissue injury

    Authors

    J. S. Pulido; I. Sugaya; J. Comstock;K. Sugaya

    Comments

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    Abbreviated Journal Title

    Graefes Arch. Clin. Exp. Ophthalmol.

    Keywords

    cornea; migration; regeneration; retina; stem cell; reelin; NEURAL STEM-CELLS; ADULT-MOUSE; DIFFERENTIATION; NEURONS; Ophthalmology

    Abstract

    Purpose Reelin is important in the guidance of neuronal stem cells in the central nervous system during normal development. We wished to determine whether reelin is expressed in the retina and cornea after injury. Methods Mice underwent laceration of their retina as well as corneal epithelial debridement. The mice were sacrificed at 3 days, and eyes were fixed and stained for reelin expression and reelin messenger ribonucleic acid (mRNA). Results In normal eyes, reelin was expressed only at very low levels in the ganglion cell layer of the retina and the endothelial cell layer of the cornea. In injured eyes, there was marked expression in reelin immunoreactivity in the retina and cornea. Reelin gene expression was seen in the retina and cornea. Conclusions Reelin is expressed during normal retinogenesis. This study shows that reelin is also upregulated following injury to the retina and cornea. The expression of reelin following injury suggests that reelin may play an important role in regulating stem cell trafficking in neuronal and nonneuronal tissues following injury similar to its role in normal organogenesis.

    Journal Title

    Graefes Archive for Clinical and Experimental Ophthalmology

    Volume

    245

    Issue/Number

    6

    Publication Date

    1-1-2007

    Document Type

    Article

    Language

    English

    First Page

    889

    Last Page

    893

    WOS Identifier

    WOS:000246594000017

    ISSN

    0721-832X

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