Keywords

Raman, Malaria, Plasmodium, Falciparum

Abstract

Raman micro-spectroscopy provides a non-destructive probe with potential applications as a diagnostic tool for cellular disorders. This study presents micro-Raman spectra of live erythrocytes infected with a malaria parasite and investigates the potential of this probe to monitor molecular changes which occur during differentiation of the parasite inside the cell. At an excitation wavelength of 633 nm the spectral bands are dominated by hemoglobin vibrations yielding information the on structure and spin state of the heme moiety. It also demonstrates the novel use of silica capillaries as a viable method for studying the erythrocytes in an environment that is much closer to their native state, thus opening the possibility of maintaining the cell in vivo for long periods to study the dynamics of the parasite's growth.

Notes

If this is your thesis or dissertation, and want to learn how to access it or for more information about readership statistics, contact us at STARS@ucf.edu

Graduation Date

2007

Semester

Summer

Advisor

Schulte, Alfons

Degree

Master of Science (M.S.)

College

College of Sciences

Department

Physics

Degree Program

Physics

Format

application/pdf

Identifier

CFE0001780

URL

http://purl.fcla.edu/fcla/etd/CFE0001780

Language

English

Release Date

September 2007

Length of Campus-only Access

None

Access Status

Masters Thesis (Open Access)

Download

Included in

Physics Commons

Share

COinS