Inflamed tissue in Crohn’s disease (CD) are continuously producing toxic oxygen metabolites leading to cellular injury and apoptosis. Here, we are evaluating the role of Mycobacterium avium subspecies paratuberculosis (MAP) in oxidative stress in CD by evaluation of lipid peroxidation and antioxidant defense activity. Specifically, we measured malondialdehyde (MDA) level and selenium-dependent glutathione peroxidase (GPx) activity in the plasma from patients and cattle infected with MAP. The level of MAP antibodies in bovine sera was determined by IDEXX kit whereas detection of MAP DNA was performed by IS900-based nPCR. A total of 42 cattle (21 infected with MAP and 21 healthy controls), 27 CD subjects, 27 of CD-healthy relatives, 66 subjects with various diseases and 34 non-related healthy subjects were investigated. Overall, GPx activity was significantly higher in MAP infected humans (0.80941±0.521) versus MAP (-ve) samples (0.42367±0.229 units/ml), P lt& 0.01. Similarly, the average of GPx activity in cattle infected with MAP was 1.59±0.65 units/ml compared to 0.46907±0.28 units/ml in healthy cattle (P lt& 0.01). Although it was not statistically significant, MDA average level was higher in MAP infected human samples versus MAP (-ve) controls (1.11±0.185 nmol/ml versus 0.805±0.151 nmol/ml, respectively). Similarly, MDA average level in CD samples that are MAP+ (1.703±0.231 nmol/ml) was higher than CD samples that are MAP (-ve) (1.429±0.187 nmol/ml). In cattle, MDA average level in MAP infected samples was significantly higher at 3.818±0.45 nmol/ml compared to 0.538±0.18 nmol/ml in healthy cattle (P lt& 0.01). Clearly, the data demonstrated that MAP infection is associated with oxidative stress and resulting in the pathophysiology of worsening of the condition of CD patients.
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Master of Science (M.S.)
College of Medicine
Molecular Biology and Microbiology
Length of Campus-only Access
Masters Thesis (Open Access)
Qasem, Ahmad, "Malondialdehyde (MDA) and Glutathione Peroxidase (GPx) are Elevated in Crohns Disease-Associated with Mycobacterium Avium Subspecies Paratuberculosis (MAP)" (2016). Electronic Theses and Dissertations, 2004-2019. 5484.