Abstract

Fluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization and propagation angle of light gives fluorescence imaging great capabilities ranging from single molecule imaging to long-term observation of living organisms. While numerous fluorescence imaging techniques have been developed over the past decades, there is always an inevitable tradeoff among the spatial resolution, imaging speed, contrast, photodamage and the total cost when it comes to choose the appropriate microscope. A main goal of my dissertation research is to develop state-of-the-art microscope systems that exhibit unprecedented performance in single-molecule fluorescence imaging and live-cell imaging for broader biomedical applications by tailoring the optical illumination beams. In details, I have designed and prototyped: 1) a highly inclined swept illumination for wide-field fluorescence microscope, which greatly improves the sectioning capability with a large field of view and ultrasensitivity; 2) dual inclined line-scan confocal microscope, which reduces photodamage while maintaining the background rejection capability compared to conventional line-scan confocal microscope; 3) a static non-diffracting light-sheet generation by controlling the spatial coherence of light emitting diodes and laser.

Notes

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Graduation Date

2020

Semester

Spring

Advisor

Han, Kyu Young

Degree

Doctor of Philosophy (Ph.D.)

College

College of Optics and Photonics

Department

Optics and Photonics

Degree Program

Optics and Photonics

Format

application/pdf

Identifier

CFE0008046; DP0023186

URL

https://purls.library.ucf.edu/go/DP0023186

Language

English

Release Date

May 2023

Length of Campus-only Access

3 years

Access Status

Doctoral Dissertation (Open Access)

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