Gold Nanoparticles have piqued interest for use in a wide range of diagnostic and therapeutic applications including biosensors, assays, vaccine development, drug delivery, and photothermal therapy. While extensive research has been conducted, the applications of gold nanoparticles have yet to see the same level of commercial success as well-established methods such as ELISA and lateral flow immunoassays. Studies analyzing the efficacy of gold nanoparticle technologies in vivo and in vitro provide seemingly conflicting results that reflect a further need for investigation into the biological response of gold nanoparticles. A large factor in the variation is the nature of the interaction between gold nanoparticles and the immune system. The immune system is among the first systems involved with the initial interaction with a foreign entity, and is comprised of several mechanisms for a variety of circumstances. While current studies have begun to provide insight regarding immunological response to gold nanoparticles, further research is still required. In this study, the interactions between gold nanoparticles and 13 different immunoglobulin isotypes from bovine, human, and murine blood were studied using dynamic light scattering, colorimetry, and UV-Vis spectroscopy. In further testing, gold nanoparticle interaction was monitored in mixed samples of immunoglobulin isotypes associated with T helper 1 and T helper 2 cell pathways to potentially observe any change in interaction as the result of a mixed sample. Preliminary results revealed a potential trend involving a greater increase in absorbance and hydrodynamic radius for isotypes associated with the T helper 1 cell related immune response. However, certain inconsistent repeatability of the results was noticed from the mixed immunoglobulin studies, and this observation seems to indicate the possibility of additional factors contributing to the immunoglobulin-gold nanoparticle interactions.
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Master of Science (M.S.)
College of Graduate Studies
Nanoscience Technology Center
Length of Campus-only Access
Masters Thesis (Open Access)
Hassan, Rick, "Interactions between Gold Nanoparticles and Immunoglobulin Isotypes" (2021). Electronic Theses and Dissertations, 2020-. 509.