Keywords

Drosophila; P5B-type ATPase; Polyamine transport; Gene editing; Substrate specificity

Abstract

In eukaryotes P-type ATPases are represented by a superfamily of membrane ion and lipid transporters divided into five subclasses. Substrates for classes 1-4 have been identified, however little is known about the substrate specificity of the P-type 5A and 5B transporters. Here we show that the Drosophila gene anne boleyn encodes a family of six P5B-type ATPases. Using a combination of RNAi, insertional mutagenesis, and CRISPR-Cas9 gene editing we show that some of these isoforms are required for polyamine transport and others are not. In addition, we show that the Drosophila P5A-type ATPase, encoded by CG6230 is not required for polyamine transport, indicating a different substrate specificity for this transporter sub-family. In summary, we provide evidence for the first time for an evolutionary conserved substrate specificity for the P5B-type ATPase sub-family of transporters.

Notes

If this is your thesis or dissertation, and want to learn how to access it or for more information about readership statistics, contact us at STARS@ucf.edu

Graduation Date

2021

Semester

Summer

Advisor

Vonkalm, Laurence

Degree

Master of Science (M.S.)

College

College of Sciences

Department

Biology

Degree Program

Biology

Format

application/pdf

Identifier

CFE0008624;DP0025355

URL

https://purls.library.ucf.edu/go/DP0025355

Language

English

Release Date

August 2021

Length of Campus-only Access

None

Access Status

Masters Thesis (Open Access)

Subjects

Adenosine triphosphatase genes; Polyamines--Metabolism; Biological transport, Active--Research; Drosophila melanogaster--Physiology; Membrane proteins--Physiological transport

Download

Included in

Biology Commons

Share

COinS
 

Accessibility Statement

This item was created or digitized prior to April 24, 2027, or is a reproduction of legacy media created before that date. It is preserved in its original, unmodified state specifically for research, reference, or historical recordkeeping. In accordance with the ADA Title II Final Rule, the University Libraries provides accessible versions of archival materials upon request. To request an accommodation for this item, please submit an accessibility request form.