Keywords

Rosocyanine, Curcumin, Borate, Nanoparticles, Antimicrobial, Antioxidant, Xanthamonas perforans, Photothermal Conversion, Optical Data Storage

Abstract

In recent years, Curcumin and its derivatives has been extensively studied as a potential bioactive molecule for treating neurodegenerative, cardiovascular, metabolic diseases, and mental illnesses. Rosocyanine, a Curcumin-Borate complex, has been used in colorimetric assays to detect the presence of boron in soil, food, and water. However, the potential medicinal benefits of Rosocyanine are yet to be explored due to its limited chemical stability under ambient conditions. Boric acid possesses anticancer, anti-inflammatory, and antioxidant properties. Therefore, it is reasonable to expect that Rosocyanine will possess enhanced antimicrobial, antiproliferative, and antioxidant properties compared to boric acid or curcumin. This study focuses on developing a stable Rosocyanine molecule using a Tannic acid (TA, a polyphenolic macromolecule)-polyvinylpyrrolidone (PVP) nanocarrier system. It is hypothesized that the TA-PVP nanocarrier will stabilize the Rosocyanine via intermolecular interactions without compromising its bioactive properties. Optical properties of Rosocyanine loaded particles under different experimental conditions was studied using UV-Vis and Fluorescence spectroscopy. Furthermore, Liquid Chromatography-Mass Spectrometry (LC-MS) and 11B Nuclear Magnetic Resonance (NMR) were used to confirm the formation of the Rosocyanine within the particles. The hydrodynamic diameter and surface charge of the Rosocyanine loaded particles were characterized through Dynamic Light Scattering and Zeta potential (DLS/Zeta) and were found to be 218 nm and - 29 mV, respectively. Overall, the material characterization results confirm that the TA-PVP nanocarrier successfully stabilized Rosocyanine in physiological conditions enabling the study of its biological properties. Antioxidant properties of the Rosocyanine loaded particles were studied using the DPPH and ABTS radical scavenging assays. Results confirmed that the antioxidant properties of the polyphenols remained intact at concentrations as low as 500 ppb. Rosocyanine’s strong optical properties allowed exploration into its potential application as a material for optical data storage and as a photothermal agent in photothermal iii therapy. The present work creates the opportunity to further study the medicinal and physicochemical properties of the nanocarrier-stabilized Rosocyanine in physiological conditions.

Completion Date

2025

Semester

Summer

Committee Chair

Swadeshmukul, Santra

Degree

Master of Science (M.S.)

College

College of Medicine

Department

Burnett School of Biomedical Sciences

Format

PDF

Release Date

8-15-2027

Document Type

Thesis

Campus Location

Orlando (Main) Campus

Subjects

Curcumin; Bioactive compounds--Biotechnology; Antioxidants--Analysis; Complex compounds--Research; Biomolecules--Therapeutic use

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Available for download on Sunday, August 15, 2027

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