Novel Murine Model for Colon Cancer: Non-Operative Trans-Anal Rectal Injection

    Authors

    M. Donigan; L. S. Norcross; J. Aversa; J. Colon; J. Smith; R. Madero-Visbal; S. A. Li; N. McCollum; A. Ferrara; J. T. Gallagher;C. H. Baker

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    Abbreviated Journal Title

    J. Surg. Res.

    Keywords

    colon cancer; orthotopic murine model; non-operative injection of cancer; cells; COLORECTAL-CANCER; OPEN COLECTOMY; MOUSE MODEL; LAPAROSCOPY; METASTASES; Surgery

    Abstract

    Background. This study was conducted to develop a modified murine model of colon cancer that is non-operative. Currently, the most accurate orthotopic murine model of colon cancer requires an invasive procedure involving cecal injection of colon cancer cells and therefore limits the ability to perform immunological studies subsequent to cecal resections. Materials and methods. Murine colon cancer (CT26) cells were injected submucosally into the distal, posterior rectum of BALB/c mice. Care was taken not to pass transmurally into the pelvic cavity. Different magnifications (10x versus 100x) were used for injection, and primary tumor growth and metastatic disease were studied. Results. In the initial study, 3/7 mice injected using 10x magnifications had notable, large tumor originating from the rectal wall, and histology revealed that all excised tumors were poorly differentiated adenocarcinoma. In the second study, 8/10 mice injected using 100x magnifications had notable tumor originating from the rectal well, and 4/8 mice had abnormal lung tissue with pathological evidence of hemorrhagic pulmonary edema. The use of 10x magnification resulted in 43% tumor take. In sharp contrast, 80% tumor take was observed with 100x magnification. The overall success of tumor take was 65% using the trans-anal rectal injection model. Conclusions. Our modified orthotopic murine model of colon cancer offers an alternative non-operative murine model for colon cancer and is less invasive than the traditional orthotopic model (i.e., cecal injection). This model may allow for more accurate investigations of inflammation and immune responses to surgical intervention without the influence of previous abdominal surgery. (C) 2009 Elsevier Inc. All rights reserved.

    Journal Title

    Journal of Surgical Research

    Volume

    154

    Issue/Number

    2

    Publication Date

    1-1-2009

    Document Type

    Article

    Language

    English

    First Page

    299

    Last Page

    303

    WOS Identifier

    WOS:000266891000020

    ISSN

    0022-4804

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