In vitro analog of human bone marrow from 3D scaffolds with biomimetic inverted colloidal crystal geometry

    Authors

    J. E. Nichols; J. Q. Cortiella; J. Lee; J. A. Niles; M. Cuddihy; S. P. Wang; J. Bielitzki; A. Cantu; R. Mlcak; E. Valdivia; R. Yancy; M. L. McClure;N. A. Kotov

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    Abstract

    in vitro replicas of bone marrow can potentially provide a Continuous source of blood cells for transplantation and serve as a laboratory model to examine human immune system dysfunctions and drug toxicology. Here we report the development of an in vitro artificial bone marrow based on a 3D scaffold with inverted colloidal crystal (ICC) geometry mimicking the structural topology of actual bone marrow matrix. To facilitate adhesion of cells, scaffolds were coated with a layer of transparent nanocomposite. After seeding with hematopoietic stern cells (HSCs), ICC scaffolds were capable of supporting expansion of CD34+ HSCs with B-lymphocyte differentiation. Three-dimensional organization was shown to be critical for production of B cells and antigen-specific antibodies. Functionality of bone marrow constructs was confirmed by implantation of matrices containing human CD34+ cells onto the backs of severe combined immunodeficiency (SCID) mice with Subsequent generation of human immune cells. 2008 (C) Elsevier Ltd. All rights reserved.

    Journal Title

    Biomaterials

    Volume

    30

    Issue/Number

    6

    Publication Date

    1-1-2009

    Document Type

    Article

    First Page

    1071

    Last Page

    1079

    WOS Identifier

    WOS:000262750300010

    ISSN

    0142-9612

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