Title

Tetranucleotide markers from the loggerhead sea turtle (Caretta caretta) and their cross-amplification in other marine turtle species

Authors

Authors

B. M. Shamblin; B. C. Faircloth; M. G. Dodd; D. A. Bagley; L. M. Ehrhart; P. H. Dutton; A. Frey;C. J. Nairn

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Conserv. Genet.

Keywords

Caretta caretta; Loggerhead turtle; Microsatellites; Cross-amplification; LOCI; DNA; Biodiversity Conservation; Genetics & Heredity

Abstract

The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea turtle. We tested primers using samples from 22 females that nested at Melbourne Beach, Florida (USA). Primer pairs yielded an average of 11.2 alleles per locus (range of 4-24), an average observed heterozygosity of 0.83 (range 0.59-0.96), and an average polymorphic information content of 0.80 (range 0.62-0.94). We also demonstrate the utility of these primers, in addition to primers for 15 loci previously described, for amplifying microsatellite loci in four additional species representing the two extant marine turtle families: olive ridley (Lepidochelys olivacea), hawksbill (Eretmochelys imbricata), green turtle (Chelonia mydas), and leatherback (Dermochelys coriacea).

Journal Title

Conservation Genetics

Volume

10

Issue/Number

3

Publication Date

1-1-2009

Document Type

Article

Language

English

First Page

577

Last Page

580

WOS Identifier

WOS:000265832000019

ISSN

1566-0621

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