Tetranucleotide markers from the loggerhead sea turtle (Caretta caretta) and their cross-amplification in other marine turtle species

    Authors

    B. M. Shamblin; B. C. Faircloth; M. G. Dodd; D. A. Bagley; L. M. Ehrhart; P. H. Dutton; A. Frey;C. J. Nairn

    Comments

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    Abbreviated Journal Title

    Conserv. Genet.

    Keywords

    Caretta caretta; Loggerhead turtle; Microsatellites; Cross-amplification; LOCI; DNA; Biodiversity Conservation; Genetics & Heredity

    Abstract

    The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea turtle. We tested primers using samples from 22 females that nested at Melbourne Beach, Florida (USA). Primer pairs yielded an average of 11.2 alleles per locus (range of 4-24), an average observed heterozygosity of 0.83 (range 0.59-0.96), and an average polymorphic information content of 0.80 (range 0.62-0.94). We also demonstrate the utility of these primers, in addition to primers for 15 loci previously described, for amplifying microsatellite loci in four additional species representing the two extant marine turtle families: olive ridley (Lepidochelys olivacea), hawksbill (Eretmochelys imbricata), green turtle (Chelonia mydas), and leatherback (Dermochelys coriacea).

    Journal Title

    Conservation Genetics

    Volume

    10

    Issue/Number

    3

    Publication Date

    1-1-2009

    Document Type

    Article

    Language

    English

    First Page

    577

    Last Page

    580

    WOS Identifier

    WOS:000265832000019

    ISSN

    1566-0621

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