Regeneration and characterization of adult mouse hippocampal neurons in a defined in vitro system

    Authors

    K. Varghese; M. Das; N. Bhargava; M. Stancescu; P. Moinar; M. S. Kindy;J. Hickman

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abstract

    Although the majority of human illnesses occur during adulthood, most of the available in vitro disease models are based upon cells obtained from embryonic/fetal tissues because of the difficulties involved with culturing adult cells. Development of adult mouse neuronal cultures has a special significance because of the abundance of transgenic disease models that use this species. in this study a novel cell culture method has been developed that supports the long-term survival and physiological regeneration of adult mouse hippocampal cells in a serum-free defined environment. In this well-defined, controlled system, adult mouse hippocampal cells survived for up to 21 days in culture. The cultured cells exhibited typical hippocampal neuronal morphology and electrophysiological properties after recovery from the trauma of dissociation, and stained positive for the expected neuronal markers. This system has great potential as an investigative tool for in vitro studies of adult diseases, the aging brain or transgenic models of age-associated disorders. (C) 2008 Elsevier B.V. All rights reserved.

    Journal Title

    Journal of Neuroscience Methods

    Volume

    177

    Issue/Number

    1

    Publication Date

    1-1-2009

    Document Type

    Article

    First Page

    51

    Last Page

    59

    WOS Identifier

    WOS:000262706300006

    ISSN

    0165-0270

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