Abbreviated Journal Title
J. Clin. Invest.
Keywords
EPITHELIAL SODIUM-CHANNEL; CORTICAL COLLECTING DUCT; K+; TRANSPORT-PROPERTIES; NAFAMOSTAT MESILATE; INDUCED PROTEIN; LOCALIZATION; HYPERKALEMIA; EXPRESSION; ACTIVATION; SUBUNIT; Medicine, Research & Experimental
Abstract
Prostasin is a serine protease present in mammalian urine that increases the activity of the epithelial sodium channel (ENaC) when the two are coexpressed in Xenopus oocytes. To determine if aldosterone, one of the principal regulators of urinary Na reabsorption by the distal nephron, affects prostasin expression, we examined prostasin mRNA and protein in a cultured mouse cortical collecting duct cell line (M-1), whole rats, and patients with primary aldosteronism. Aldosterone treatment of M-1 cells substantially increased prostasin expression and stimulated Na-22 uptake. Urinary excretion of prostasin in rats that were infused with aldosterone likwise increased by similar to4-fold when compared with the vehicle-infused rats. Finally, urinary excretion of prostasin in patients with primary aldosteronism was substantially increased when compared with normal patients. Adrenalectomy reduced urinary prostasin excretion to control levels, whereas urinary prostasin levels were not altered in patients undergoing surgery, for other reasons. In patients with primary aldosteronism, reduction in the urinary excretion of prostasin correlated with the increase in the urinary Na/K ratio. These Findings, together with our previous report that prostasin activates the amiloride-sensitive Na currents through ENaC, demonstrate that prostasin regulates Na balance in vivo by virtue of its heightened expression in the presence of aldosterone.
Journal Title
Journal of Clinical Investigation
Volume
109
Issue/Number
3
Publication Date
1-1-2002
Document Type
Article
DOI Link
Language
English
First Page
401
Last Page
408
WOS Identifier
ISSN
0021-9738
Recommended Citation
Narikiyo, Takefumi; Kitamura, Kenichiro; Adachi, Masataka; Miyoshi, Taku; Iwashita, Kozo; Shiraishi, Naoki; Nonoguchi, Hiroshi; Chen, Li-Mei; Chai, Karl X.; Chao, Julie; and Tomita, Kimio, "Regulation of prostasin by aldosterone in the kidney" (2002). Faculty Bibliography 2000s. 3374.
https://stars.library.ucf.edu/facultybib2000/3374
Comments
Authors: contact us about adding a copy of your work at STARS@ucf.edu