Title

Molecular epidemiology of Mycobacterium avium subsp paratuberculosis: Evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis

Authors

Authors

A. S. Motiwala; M. Strother; A. Amonsin; B. Byrum; S. A. Naser; J. R. Stabel; W. P. Shulaw; J. P. Bannantine; V. Kapur;S. Sreevatsan

Comments

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Abbreviated Journal Title

J. Clin. Microbiol.

Keywords

FRAGMENT-LENGTH-POLYMORPHISM; POLYMERASE-CHAIN-REACTION; RESTRICTION-ENDONUCLEASE ANALYSIS; CROHNS-DISEASE; FECAL CULTURE; TUBERCULOSIS COMPLEX; DNA HYBRIDIZATION; JOHNES-DISEASE; IS900; PCR; Microbiology

Abstract

The objectives of this study were to understand the molecular diversity of animal and human strains of Mycobacterium avium subsp. paratuberculosis isolated in the United States and to identify M. avium subsp. paratuberculosis-specific diagnostic molecular markers to aid in disease detection, prevention, and control. Multiplex PCR of IS900 integration loci (MPIL) and amplified fragment length polymorphism (AFLP) analyses were used to fingerprint M. avium subsp. paratuberculosis isolates recovered from animals (n = 203) and patients with Crohn's disease (n = 7) from diverse geographic localities. Six hundred bacterial cultures, including M. avium subsp. paratuberculosis (n 303), non-M. avium subsp. paratuberculosis mycobacteria (n = 129), and other nonmycobacterial species (n 168), were analyzed to evaluate the specificity of two IS900 integration loci and a newly described M. avium subsp. paratuberculosis-specific sequence (locus 251) as potential targets for the diagnosis of M. avium subsp. paratuberculosis. MPIL fingerprint analysis revealed that 78% of bovine origin M. avium subsp. paratuberculosis isolates clustered together into a major node, whereas isolates from human and ovine sources showed greater genetic diversity. MPIL analysis also showed that the M. avium subsp. paratuberculosis isolates from ovine and bovine sources from the same state were more closely associated than were isolates from different geographic regions, suggesting that some of the strains are shared between these ruminant species. AFLP fingerprinting revealed a similar pattern, with most isolates from bovine sources clustering into two major nodes, while those recovered from sheep or humans were clustered on distinct branches. Overall, this study identified a high degree of genetic similarity between M. avium subsp. paratuberculosis strains recovered from cows regardless of geographic origin. Further, the results of our analyses reveal a relatively higher degree of genetic heterogeneity among M. avium subsp. paratuberculosis isolates recovered from human and ovine sources.

Journal Title

Journal of Clinical Microbiology

Volume

41

Issue/Number

5

Publication Date

1-1-2003

Document Type

Article

Language

English

First Page

2015

Last Page

2026

WOS Identifier

WOS:000182934500034

ISSN

0095-1137

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