An mRNA and DNA co-isolation method for forensic casework samples

    Authors

    M. Alvarez; J. Juusola;J. Ballantyne

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    Anal. Biochem.

    Keywords

    mRNA; DNA; co-extraction; body fluid identification; SEMEN STAINS; DRIED BLOOD; CELLS; EXTRACTION; TISSUES; PCR; IDENTIFICATION; REAGENT; RIG; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical

    Abstract

    RNA analysis is expected to play an increasingly important role in the area of biomolecular forensic analysis. For example, mRNA expression analysis performed on a total RNA sample isolated from a biological stain may be used to identify the nature of the tissue(s) comprising the stain. Many of the physiological stains encountered at crime scenes involve heterogeneous mixtures of different body fluids (e.g., semen and saliva, semen and vaginal secretions). Separate sampling of these mixed stains from different "geographical" locations of the stains to isolate DNA and RNA could result in a misleading estimate of the ratio of the body fluids present and, in extreme cases, even fail to detect one of the contributors. Thus, a prerequisite for the use of mRNA expression profiling in routine forensic analysis is the ability to co-extract DNA and RNA from the same stain. This article describes an optimized method that was specifically developed to co-extract mRNA and DNA from the same physiological stain and that appears to be sufficiently sensitive and robust for routine forensic use. (C) 2004 Elsevier Inc. All rights reserved.

    Journal Title

    Analytical Biochemistry

    Volume

    335

    Issue/Number

    2

    Publication Date

    1-1-2004

    Document Type

    Article

    Language

    English

    First Page

    289

    Last Page

    298

    WOS Identifier

    WOS:000225502800014

    ISSN

    0003-2697

    Share

    COinS