An investigation on the analytical potential of polymerized liposomes bound to lanthanide ions for protein analysis

    Authors

    M. Santos; B. C. Roy; C. Goicoechea; A. D. Campiglia;S. Mallik

    Comments

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    Abbreviated Journal Title

    J. Am. Chem. Soc.

    Keywords

    DYE-BINDING; INTERFACIAL CATALYSIS; SENSITIVE METHOD; LIPID-MEMBRANES; VESICLES; SPECTROSCOPY; ASSAY; MICROARRAYS; FRACTIONS; DEVICES; Chemistry, Multidisciplinary

    Abstract

    We present a promising approach to protein sensing based on Eu3+ ions incorporated into polymerized liposomes. The sensitization of Eu3+ is accomplished with 5-aminosalicylic acid, which provides energy transfer for a stable reference signal and a wide wavelength excitation range free from protein interference. The lipophilic character of polymerized liposomes provides the appropriate platform for protein interaction with the lanthanide ion. Quantitative analysis is based on the linear relationship between the luminescence signal of Eu3+ and protein concentration. Because no spectral shift of the lanthanide luminescence is observed upon protein interaction, qualitative analysis is based on the luminescence lifetime of polymerized liposomes. This parameter, which changes significantly upon protein - liposome interaction, follows a well-behaved single-exponential decay that might be useful for protein identification.

    Journal Title

    Journal of the American Chemical Society

    Volume

    126

    Issue/Number

    34

    Publication Date

    1-1-2004

    Document Type

    Article

    Language

    English

    First Page

    10738

    Last Page

    10745

    WOS Identifier

    WOS:000223559100055

    ISSN

    0002-7863

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