A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens

    Authors

    M. Le Puil; J. P. Biggerstaff; B. L. Weidow; J. R. Price; S. A. Naser; D. C. White;R. S. Alberte

    Comments

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    Abbreviated Journal Title

    J. Microbiol. Methods

    Keywords

    deconvolution; spectral imaging; confocal microscopy; pathogens; FISH; OLIGONUCLEOTIDE PROBES; CLOSTRIDIUM-DIFFICILE; FLOW-CYTOMETRY; BACTERIA; DISEASE; CELLS; PARATUBERCULOSIS; IDENTIFICATION; HYBRIDIZATION; MICROBIOLOGY; Biochemical Research Methods; Microbiology

    Abstract

    A novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. It allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in suspension and provided quantitative results. (c) 2006 Elsevier B.V. All rights reserved.

    Journal Title

    Journal of Microbiological Methods

    Volume

    67

    Issue/Number

    3

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    597

    Last Page

    602

    WOS Identifier

    WOS:000242252200022

    ISSN

    0167-7012

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