Title

A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens

Authors

Authors

M. Le Puil; J. P. Biggerstaff; B. L. Weidow; J. R. Price; S. A. Naser; D. C. White;R. S. Alberte

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

J. Microbiol. Methods

Keywords

deconvolution; spectral imaging; confocal microscopy; pathogens; FISH; OLIGONUCLEOTIDE PROBES; CLOSTRIDIUM-DIFFICILE; FLOW-CYTOMETRY; BACTERIA; DISEASE; CELLS; PARATUBERCULOSIS; IDENTIFICATION; HYBRIDIZATION; MICROBIOLOGY; Biochemical Research Methods; Microbiology

Abstract

A novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. It allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in suspension and provided quantitative results. (c) 2006 Elsevier B.V. All rights reserved.

Journal Title

Journal of Microbiological Methods

Volume

67

Issue/Number

3

Publication Date

1-1-2006

Document Type

Article

DOI Link

http://dx.doi.org/10.1016/j.mimet.2006.05.019

Language

English

First Page

597

Last Page

602

WOS Identifier

WOS:000242252200022

ISSN

0167-7012

Recommended Citation

"A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens" (2006). Faculty Bibliography 2000s. 6330.
https://stars.library.ucf.edu/facultybib2000/6330