Enhanced imaging and accelerated photothermalysis of A549 human lung cancer cells by gold nanospheres

    Authors

    X. Liu; M. C. Lloyd; I. V. Fedorenko; P. Bapat; T. Zhukov;Q. Huo

    Comments

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    Abbreviated Journal Title

    Nanomedicine

    Keywords

    cancer; gold nanoparticles; laser; photothermal therapy; photothermalysis; viability; NANOPARTICLE PROBES; THERAPY; NANORODS; NANOPHOTOTHERMOLYSIS; Biotechnology & Applied Microbiology; Nanoscience & Nanotechnology

    Abstract

    Background & aims: Gold nanoparticles are excellent photon-thermal energy converters. The purpose of this work was to investigate the influence of gold nanoparticles on the photothermalysis of A549 lung tumor cells. Materials & methods: A549 lung tumor cells were exposed to goat antihuman immunoglobulin (Ig)G-conjugated gold nanospheres (40 nm) and were then imaged under a dark-field microscope. The live cells were then subjected to photoirradiation using a 633-nm laser at different power levels. The viability of tumor cells under laser irradiation was monitored by confocal microscopy using a viability-assay kit. Results & discussion: The death rates of A549 lung tumor cells after gold nanoparticle exposure increased significantly under laser irradiation. The maximum initial cell death rate was observed at a laser power level of 3.75 mW, with the initial deactivation rate accelerated by a factor of 6.6 and a total loss of 92% of cell viability. Conclusion: This work demonstrated potential applications of gold nanospheres as both imaging probes and enhancing agents for photothermal therapy of cancer.

    Journal Title

    Nanomedicine

    Volume

    3

    Issue/Number

    5

    Publication Date

    1-1-2008

    Document Type

    Article

    Language

    English

    First Page

    617

    Last Page

    626

    WOS Identifier

    WOS:000260291400010

    ISSN

    1743-5889

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