Solid-liquid extraction fluorescence line narrowing spectroscopy of fluoroquinolones in aqueous samples

    Authors

    S. J. Yu; D. G. Gomez;A. D. Campiglia

    Comments

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    Abbreviated Journal Title

    Appl. Spectrosc.

    Keywords

    fluoroquinolones; solid-liquid extraction; fluorescence line narrowing; spectroscopy; FLNS; THIN-LAYER-CHROMATOGRAPHY; SHPOLSKII SPECTROSCOPY; SURFACE-WATER; WASTE-WATER; ANTIBIOTICS; QUINOLONES; ADDUCTS; URINE; SERUM; Instruments & Instrumentation; Spectroscopy

    Abstract

    We present a simple, selective, and sensitive method for the analysis of fluoroquinolones in water samples without previous separation. Octadecyl silica membranes are used with the dual purpose of sample preconcentration and solid substrate for fluorescence line narrowing spectroscopy. Measurements at liquid helium temperature (4.2 K) are easily made with the aid of a cryogenic fiber-optic probe. The entire procedure takes less than 15 minutes per sample and it consumes only 100 mu L of organic solvent. Unambiguous fluoroquinolone determination is accomplished via wavelength-time matrices, which provide simultaneous information on spectral peak purity and fluorescence lifetime. We show that 10 mL of water sample are enough to detect analyte concentrations at parts-per-billion levels. The potential of this approach for the analysis of real world samples is demonstrated with the analysis of a synthetic mixture of seven fluoroquinolones spiked in a heavily contaminated water sample.

    Journal Title

    Applied Spectroscopy

    Volume

    60

    Issue/Number

    10

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    1174

    Last Page

    1180

    WOS Identifier

    WOS:000241144000012

    ISSN

    0003-7028

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